Enzyme Activity Essay Sample free essay sample

We performed these experiments to detect the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in assorted provinces and per centums. and observed the soaking up values of the enzyme-substrate relationship at different concentrations. Our consequences show that the more substrate available. the quicker the reaction will go on except in one trial. which might intend that a balanced concentration of the two is most good. Introduction The aims of these experiments were to detect the effects of the enzyme-substrate relationships and to enter our findings. Enzymes increase the rate of reactions by take downing the energy needed to trip the reaction ( McNeil et al. 2013 ) . Enzymes will work with substrates to bring forth reactions and merchandises and they will adhere together at an active site. They will merely bond to with peculiar molecules and environmental factors can besides impact their productiveness. We will write a custom essay sample on Enzyme Activity Essay Sample or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page They are proteins. and proteins are made up of many aminic acids ( Brian et al. 2013 ) . We used the enzyme catalase that occurs of course in many beings to analyze the qualitative and quantitative consequences of enzymatic activity. My hypothesis is that the findings in these experiments will demo that the enzyme catalase will increase the rate of reaction with the substrate. Methods In Activity 1 Procedure A. we had four trial tubings filled with different constituents. The tabular array below shows each tube’s constituents. In each trial tubing. we added 5. 0 mL 3 % H2O2. We recorded initial observations and checked often for alterations. Table 1.Tube # | Contents|1| 1 milliliter H2O|2| ? ? ?† murphy regular hexahedron |3| 1 milliliter Enz. |4| 1 milliliter Enz boiled for 5 proceedingss. so cooled| In Activity 1 Procedure B. we prepared two more trial tubing with different substrates. In each empty tubing we put 1 milliliter of enzyme. To that. we added the same substrate with different per centum degrees. What we added to the trial tubing is depicted in the chart below. We recorded our observations of these tubings and compared observations ab initio with those of proceedingss 4-5. Table 2.Tube # | Contentss |A| 1 milliliter Enz. + 5. 0 mL1. 5 % H2O2|B| 1 milliliter Enz. + 5. 0 milliliter. 75 % H2O2| In Activity 2 Procedure C. we filtered the catalase used in Procedures A and B with # 4 filter paper. We made a black solution without the catalase and another with it to be compared in the spectrophotometer. The contents of the space and cuvette # 1 are shown below. We observed optical density degrees at 470 nanometers and measured the space to deduct its values from those of cuvette # 1. We measured the optical density every minute for 5 proceedingss and recorded our observations. After the 5 proceedingss we removed them and observed differences. Table 3.Cuvette # | Contentss |Blank | 6. 0 mL dH2O + . 100 uL guaiacol + . 150 uL H2O2|1| 1. 0 mL catalase + 5. 0 mL dH2O + . 100 uL guaiacol + . 150 uL H2O2| In Activity 2 Procedure D. we followed the same processs as we did in Procedure C ; nevertheless. the contents of the space and cuvette were changed. The alterations are shown in the tabular array below. Table 4.Cuvette # | Contentss |Blank | 5 milliliter dH2O + . 100 uL guaiacol + . 300 uL H2O2|1| 1. 0 mL catalase + 4. 0 mL dH2O + . 100 uL guaiacol + . 300 uL H2O2| These methods came from the Biology 183 Introductory II Lab Manual. ConsequencesThe presence of an enzyme speeds up chemical reactions and is affected by the concentration of the substrate. We found that the consequences of reaction were much greater and happened faster with the presence of a greater sum of substrate and enzyme until there was excessively much substrate in relation to enzyme. In Activity 1 Procedure A. we found that the more available substrate nowadays. the faster the reaction would go on. More merchandise was observed when there was increased substrate surface country. The tabular array of consequences is depicted below. Table 5.Tube # | Contents| What Happened? |1| 1 milliliter H2O + 5. 0 mL 3 % H2O2| No reaction |2| ? ? ?† murphy regular hexahedron + 5. 0 mL 3 % H2O2| Bubbling and foaming occurred but non much| 3| 1 milliliter Enz. + 5. 0 mL 3 % H2O2| More froth and bubbles than in previous| 4| 1 milliliter Enz boiled for 5 proceedingss. so cooled + 5. 0 mL 3 % H2O2| Barely any mark of reaction| In Activity 1 Procedure B. we found that the concentration of substrate affects the activity of the enzyme. The solution with a higher concentration of substrate produced greater consequences. Table 6.Tube # | Contentss | What Happened? |A| 1 milliliter Enz. + 5. 0 mL1. 5 % H2O2| Foamed and bubbled rapidly ; much more than B| B| 1 milliliter Enz. + 5. 0 milliliter. 75 % H2O2| Foamed and bubbled less and at a slower rate. | In Activity 2 Procedure C. we discovered that our solution with catalase formed merchandises and the solution without did non. The spectrophotometer collected informations for us to demo this. Table 7.Cuvette # | Contentss | What Happened? |Blank | 6. 0 mL dH2O + . 100 uL guaiacol + . 150 uL H2O2| No change| 1| 1. 0 mL catalase + 5. 0 mL dH2O + . 100 uL guaiacol + . 150 uL H2O2| The colour of the solution changed. It got darker. | Table 8.Absorbance Data Collection of Cuvette Containing Catalase| Time ( min ) | 0| 1| 2| 3| 4| 5|Optical density at 470 nm| 1. 200 A| 1. 449 A| 1. 673 A| 1. 872 A| 2. 056 A| 2. 223 A| In Activity 2 Procedure D. our consequences showed us that the concentration of substrate can be excessively high for a same merchandise in enzyme activity when compared with the tabular array in Procedure C. A tabular array of the consequences of Procedure D and a graph comparison Procedures C and D are depicted below. Table 9.Cuvette # | Contents| What Happened? |Blank | 5 milliliter dH2O + . 100 uL guaiacol + . 300 uL H2O2| No Change| 1| 1. 0 mL catalase + 4. 0 mL dH2O + . 100 uL guaiacol + . 300 uL H2O2| The colour of the solution changed. Got darker but non every bit dark as Cuvette 1 in Procedure C. | Table 10.Absorbance Data Collection of Cuvette Containing Catalase| Time ( min ) | 0| 1| 2| 3| 4| 5|Optical density at 470 nm| . 428 A| . 673 A| . 876 A| 1. 063 A| 1. 228 A| 1. 377 A| Figure 1. Figure 1. DiscussionThe consequences found in our experiments supported the hypothesis that enzymes would increase the rate of reaction. In one instance. nevertheless. it was found that if the concentration of substrate is excessively high. the enzymatic relationship will be thrown away. We observed noticeable merchandises more rapidly with the enzyme nowadays in both experiments in Activity 1. Our experiment in Activity 2 Procedure D shows that with a higher per centum of substrate. less visible radiation was absorbed. This was unexpected because we thought that with more substrate. the reaction would take topographic point more rapidly. Our findings supported that enzymes increase the rate at which reactions occur. If this experiment was repeated. we might acquire a few fluctuations in consequences. The measurings of some substances might hold been a small off and the clip that we took to set some of the cuvettes might hold been excessively long and affected the consequences.